Coding
Part:BBa_K4482013:Design
Designed by: Mustafo Mustafokulov Group: iGEM22_NYC_Empire_State (2022-10-09)
HIR eGFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 963
Illegal PstI site found at 2464
Illegal PstI site found at 2730 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 963
Illegal PstI site found at 2464
Illegal PstI site found at 2730
Illegal NotI site found at 28 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 963
Illegal BglII site found at 1532
Illegal BamHI site found at 1878
Illegal BamHI site found at 4154
Illegal XhoI site found at 286
Illegal XhoI site found at 2043
Illegal XhoI site found at 3056 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 963
Illegal PstI site found at 2464
Illegal PstI site found at 2730 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 963
Illegal PstI site found at 2464
Illegal PstI site found at 2730
Illegal NgoMIV site found at 14
Illegal NgoMIV site found at 2116 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1744
Illegal BsaI site found at 2054
Illegal BsaI.rc site found at 221
Illegal BsaI.rc site found at 796
Illegal BsaI.rc site found at 1252
Illegal BsaI.rc site found at 1574
Illegal BsaI.rc site found at 3151
Illegal BsaI.rc site found at 3326
Illegal SapI site found at 267
Illegal SapI.rc site found at 3333
Design Notes
We added the sequence for eGFP to the C terminus of the plasmid coding for human insulin receptor.
Source
This part was ordered online from AddGene (catalog number 22286)